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Updated: Jun 27, 2026

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
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Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

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Oxygen generating scaffolds for enhancing engineered tissue survival.

Se Heang Oh1, Catherine L Ward, Anthony Atala

  • 1Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157, USA.

Biomaterials
|November 21, 2008
PubMed
Summary
This summary is machine-generated.

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Incorporating oxygen-generating biomaterials into tissue engineering scaffolds enhances cell viability under low-oxygen conditions. This innovation may improve cell survival during the critical neovascularization phase in engineered tissues.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Vascularization is a critical challenge for clinically applicable engineered tissues.
  • Existing methods using growth factors have not sufficiently accelerated angiogenesis.
  • Oxygen diffusion limitations hinder the engineering of large tissue implants.

Purpose of the Study:

  • To investigate sustained oxygen release from biomaterials in tissue engineering scaffolds.
  • To assess the impact of oxygen-generating biomaterials on cell viability and construct integrity.
  • To determine if oxygen-generating materials can overcome hypoxic limitations in engineered tissues.

Main Methods:

  • Incorporation of calcium peroxide-based oxygen-generating particles into Poly(d,l-lactide-co-glycolide) (PLGA) scaffolds.

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Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
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Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

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Last Updated: Jun 27, 2026

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
13:21

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Published on: April 6, 2022

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures
11:56

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

Published on: January 6, 2010

Procedure for Lung Engineering
12:50

Procedure for Lung Engineering

Published on: March 8, 2011

  • Design of scaffolds for sustained oxygen release over 10 days while maintaining mechanical integrity.
  • Incubation of scaffolds under hypoxic conditions to evaluate oxygen levels and cell viability.

    • Main Results:

    • Scaffolds with oxygen-generating materials maintained elevated oxygen levels under hypoxic conditions.
    • These biomaterials successfully extended cell viability and growth in hypoxic environments.
    • The scaffolds demonstrated sufficient mechanical integrity throughout the study.

    Conclusions:

    • Oxygen-generating biomaterials can provide sustained oxygen release, enhancing cell survival in engineered tissues.
    • This approach may be crucial for improving cell survivability during neovascularization after implantation.
    • Such scaffolds offer a promising solution for overcoming oxygen diffusion limitations in large tissue engineering constructs.