Related Experiment Video
Updated: Jun 27, 2026

Quantitative Fundus Autofluorescence for the Evaluation of Retinal Diseases
Published on: March 11, 2016
Autofluorescence imaging of pingueculae
C A Utine1, S Tatlipinar, M Altunsoy
1Yeditepe University Eye Hospital, Sakir Kesebir sokak No:28, Balmumcu Besiktas, 34345, Istanbul, Turkey. cananutine@gmail.com
Aims:
To analyse the autofluoresence (AF) properties of pingueculae and compare the size of AF with the extent of the visible lesion.
Methods:
Forty eyes of 23 patients with pingueculae were included in the study. AF images were obtained using an HRA2 confocal scanning laser ophthalmoscope; anterior segment photographies were obtained using TRC-50IX, IMAGEnet 2000 Digital Imaging System. The AF characteristics of pingueculae were analysed. The extent of visible lesion in anterior segment photography and AF image was compared.
Results:
AF images revealed well-defined hyper-autofluorescence in the area of pinguecula, which was greater than the extent of visible pinguecula in the slit-lamp examination, in 40 of 56 lesions (71.4%). In none of the eyes was the hyperautofluorescent area smaller than the extent of visible lesion.
Conclusion:
Pingueculae display hyperautofluorescence in AF imaging. The real size of pingueculae may be estimated by its AF characteristics, which is mostly larger than the visible lesion.
More Related Videos
10:39Improved Lipofuscin Models and Quantification of Outer Segment Phagocytosis Capacity in Highly Polarized Human Retinal Pigment Epithelial Cultures
Published on: April 14, 2023
11:01Automated Imaging and Analysis for the Quantification of Fluorescently Labeled Macropinosomes
Published on: August 24, 2021