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Updated: Jun 27, 2026

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
Published on: December 17, 2013
Assessing the antiviral activity of HIV-1-specific cytotoxic T lymphocytes
1Division of Infectious Diseases, Department of Medicine, AIDS Institute, Geffen School of Medicine, UCLA Medical Center, Los Angeles, CA, USA.
Abstract:
Currently available assays for detecting HIV-1-specific cytotoxic T lymphocytes (CTL) are remarkable for their technical ease, sensitivity, and precision of measurement. However, it is becoming increasingly apparent that CTL responses vary in their antiviral activity at the clonal level. These assays do not reveal the antiviral efficacies of the CTL they detect. Thus, an experimental approach that has been developed for this purpose has been the coculture of CTL with HIV-1-infected cells, to measure the impact of the CTL on viral replication. Data from such experiments can be useful to elucidate the contribution of viral and CTL factors to the antiviral efficiency of CTL.

