Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Pass-through photon-based biomedical transillumination.

Paulino Vacas-Jacques1, Gonzalo Paez, Marija Strojnik

  • 1Centro de Investigaciones en Optica, Apartado Postal 1-948, 37000, Leon, Guanajuato, Mexico. pvj@cio.mx

Journal of Biomedical Optics
|November 22, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Harmonic-Selective Gaussian Filtering for Morphology and Timing Preservation in PPG Signals.

Sensors (Basel, Switzerland)·2026
Same author

Thoracic electrical bioimpedance in a Lissajous plane: Pre-post smoking changes and PCA of ellipse metrics.

Physiological measurement·2026
Same author

Rotationally shearing interferometer for exoplanet detection: mathematical derivation, theory, and simulation.

Optics express·2025
Same author

Thermal Bed Design for Temperature-Controlled DNA Amplification Using Optoelectronic Sensors.

Sensors (Basel, Switzerland)·2024
Same author

Rotational shearing interferometer and wavefront angular derivative.

Optics express·2023
Same author

Electrical bioimpedance analysis and comparison in biological tissues through crystalloid solutions implementation.

Biosensors & bioelectronics·2023
Same journal

Segmentation-guided photon pooling enables robust single-cell analysis and fast fluorescence lifetime imaging microscopy.

Journal of biomedical optics·2026
Same journal

Method of spatial scanning of modulated laser radiation for outline imaging of interphalangeal joints.

Journal of biomedical optics·2026
Same journal

Multimodal optical imaging for the assessment of the teratogenic effects of ethanol on zebrafish development.

Journal of biomedical optics·2026
Same journal

Fluorescence properties of collagen types I-V: a comprehensive study of spectral and lifetime characteristics.

Journal of biomedical optics·2026
Same journal

Spectral dependence of lipofuscin fluorescence lifetimes revealed by FLIM with a superconducting nanowire single-photon detector.

Journal of biomedical optics·2026
Same journal

Building the future of biophotonics through experiential education and seasonal schools.

Journal of biomedical optics·2026
See all related articles

This study validates a new biomedical imaging technique using pass-through photons in interferometric transillumination. The method shows promise for tissue characterization and diagnostic imaging applications.

Area of Science:

  • Biomedical Optics
  • Medical Imaging Physics

Background:

  • Partial coherence interferometry is explored for transillumination.
  • Pass-through (ballistic) photons offer potential for non-invasive tissue analysis.

Purpose of the Study:

  • To present the mathematical foundation and experimental validation of a pass-through photon technique for biomedical analysis.
  • To establish feasibility for tissue characterization and diagnostic imaging.

Main Methods:

  • Utilized a partial coherence interferometric transillumination setup.
  • Employed the complex material coherence function concept.
  • Developed a normalization procedure for in vitro and in vivo measurements.

Main Results:

  • Demonstrated implementation sensitivity to tissue properties, power, source characteristics, and detection electronics.

More Related Videos

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
10:56

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Published on: August 1, 2019

Related Experiment Videos

Last Updated: Jun 27, 2026

Fluorescence Lifetime Macro Imager for Biomedical Applications
06:01

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue
07:48

High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Published on: September 30, 2022

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
10:56

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Published on: August 1, 2019

  • Obtained sample coherence function of a tissue phantom, confirming exponential attenuation.
  • Determined scattering coefficients with good agreement between theory and experiment.

    • Conclusions:

    • Pass-through photon-based transillumination is feasible for selected biomedical applications.
    • The technique shows potential for tissue characterization and diagnostic imaging.
    • A normalization procedure effectively cancels non-tissue-related quantities.