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Updated: Jun 27, 2026
Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Published on: July 9, 2008
Antibody-labeled fluorescence imaging of dendritic cell populations in vivo
Ryan J Cummings1, Soumya Mitra, Edith M Lord
1University of Rochester Medical Center ,Department of Microbiology and Immunology, Rochester, New York 14642, USA.
Abstract:
We report an optical molecular imaging technique that exploits local administration of fluorophore-conjugated antibodies and confocal fluorescence microscopy to achieve high-contrast imaging of host cell populations in normal and tumor tissue in living mice. The method achieves micron-scale spatial resolution to depths greater than 100 mum. We illustrate the capabilities of this approach by imaging two dendritic cell populations in the skin and normal and tumor vasculature in vivo.
