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Updated: Jun 27, 2026

Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
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Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery.

Lingfeng Yu1, Samarendra Mohanty, Gangjun Liu

  • 1University of California, Irvine, Beckman Laser Institute, Irvine, California 92612, USA. yulingfeng@gmail.com

Journal of Biomedical Optics
|November 22, 2008
PubMed
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This study introduces a quantitative phase laser microsurgery system for real-time analysis of cell damage. The advanced method allows precise measurement of alterations to transparent biological structures like red blood cells (RBCs).

Area of Science:

  • Biophysics
  • Cell Biology
  • Optical Microscopy

Background:

  • Laser microbeams enable minimally invasive manipulation of subcellular structures for biological studies.
  • Conventional phase-contrast microscopy lacks the ability to quantitatively assess laser-induced damage to transparent cells.

Purpose of the Study:

  • To develop and present a novel quantitative phase laser microsurgery system.
  • To enable real-time evaluation of dynamic phase changes during laser microsurgery.
  • To allow absolute quantitation of localized damage to transparent phase objects.

Main Methods:

  • Combining laser microirradiation with short-coherence interference microscopy.
  • Utilizing quantitative phase imaging to monitor cellular changes.
  • Applying the system to red blood cells (RBCs) for microsurgery.

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Last Updated: Jun 27, 2026

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Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture

Published on: February 23, 2018

Cell Membrane Repair Assay Using a Two-photon Laser Microscope
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Published on: January 2, 2018

Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair
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Imaging Cell Membrane Injury and Subcellular Processes Involved in Repair

Published on: March 24, 2014

Main Results:

  • Real-time quantitative phase images and dynamic phase changes during laser microsurgery of RBCs were successfully evaluated.
  • The system demonstrated the ability to perform absolute quantitation of localized alterations to cell membranes and intracellular structures.
  • This level of quantitative analysis surpassed the capabilities of conventional phase-contrast microscopy.

Conclusions:

  • The quantitative phase laser microsurgery system offers a powerful tool for studying structure-function relationships in biological systems.
  • It enables precise, real-time measurement of laser-induced damage in transparent cells.
  • This technology advances the field of cell biology by providing new quantitative insights into cellular manipulation and damage assessment.