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Nonlinear optical microscopy of the bronchus.

Shuangmu Zhuo¹, Jianxin Chen, Biying Yu

¹Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fuzhou 350007, P. R. China.

Journal of Biomedical Optics

|November 22, 2008

Summary

Nonlinear optical microscopy (NLOM) effectively images bronchial tissue, revealing microstructural details and cellular metabolism. This technique shows promise for in vivo diagnosis and monitoring of bronchial diseases.

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Area of Science:

Biomedical Optics
Pulmonary Medicine
Microscopy

Background:

The bronchus is susceptible to early pathological changes due to carcinogen exposure.
Assessing these early changes is crucial for physiological studies and diagnosing bronchial diseases.

Purpose of the Study:

To evaluate the efficacy of nonlinear optical microscopy (NLOM) for imaging mouse bronchial tissue.
To explore NLOM's capability in assessing tissue biomorphology, biochemistry, and metabolic state.

Main Methods:

Utilized NLOM to image intact mouse bronchial tissue leveraging intrinsic nonlinear optical contrast.
Employed two-photon ratiometric redox fluorometry to analyze mitochondrial signals (NADH, NADPH, Fp).
Measured second-harmonic signal depth-dependent decay to assess stromal optical properties.

Main Results:

NLOM successfully imaged bronchial microstructural components, providing quantitative biomorphological and biochemical data.
NLOM enabled assessment of epithelial cell and chondrocyte metabolic states via redox fluorometry.
The technique provided quantitative stromal optical property information correlated with tissue health.

Conclusions:

NLOM is a powerful tool for detailed imaging and quantitative analysis of bronchial tissue.
NLOM's ability to assess cellular metabolism and stromal properties aids in understanding disease states.
The development of portable nonlinear optical endoscopy suggests NLOM's potential for in vivo clinical diagnosis and monitoring of bronchial diseases.