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MAPPIT: a versatile tool to study cytokine receptor signalling
Irma Lemmens1, Sam Lievens, Jan Tavernier
1Department of Medical Protein Research, VIB, Ghent University, Ghent, Belgium.
Abstract:
MAPPIT (mammalian protein-protein interaction trap) is a cytokine receptor-based two-hybrid method that operates in intact mammalian cells. A bait is fused C-terminally to a STAT (signal transducer and activator of transcription) recruitment-deficient receptor, whereas the prey is linked to functional STAT-binding sites. When bait and prey interact a ligand-dependent complementation of the STAT recruitment deficiency occurs, leading to activation of a STAT-responsive reporter. MAPPIT is very well suited to study protein interactions involving activated cytokine receptors as the technique allows modification of the bait protein in a physiologically optimal environment.
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