Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Development of Blood Vessels

Development of Blood Vessels

The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...

Development of the Heart

Development of the Heart

The development of the human heart, a crucial organ, commences from the mesoderm on the 18th or 19th day after fertilization. This process initiates in the cardiogenic area, a group of mesodermal cells at the embryo's head end, which evolves into elongated strands known as cardiogenic cords. These cords undergo a transformation to form hollow-centered endocardial tubes.
As the embryo undergoes lateral folding, these paired tubes approach each other, merging into a single primitive heart tube by...

Coronary Circulation

Coronary Circulation

The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively.
Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...

Coronary Artery Disease I: Introduction

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...

Physiology of the Heart: The Cardiac Cycle

Physiology of the Heart: The Cardiac Cycle

The cardiac cycle describes the events from one heartbeat to the next. It includes three main phases: diastole, atrial systole, and ventricular systole, all driven by changes in chamber pressures and the function of heart valves.
Diastole: The Relaxation Phase
During diastole, all four heart chambers relax. The atrioventricular (AV) valves open, and the semilunar valves close. This phase sees the lowest chamber pressures, promoting ventricular filling. Venous blood enters the heart through the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Host Genetic Regulation of NLRP3 Inflammasome Cytokines Reveals Immune and Vascular Pathways in HIV.

medRxiv : the preprint server for health sciences·2026

Same author

The response to kidney injury is epigenetically regulated through the activation of bivalent genes.

American journal of physiology. Renal physiology·2026

Same author

Serum response factor is essential for endometrial function and prevention of inflammatory fibrosis.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

ECM-Focused Proteomic Analysis of Ear Punch Regeneration in <i>Acomys cahirinus</i>.

Journal of proteome research·2025

Same author

A mouse model engineered to spatiotemporally control Cre expression in progesterone receptor positive cells†.

Biology of reproduction·2025

Same author

New Tools for Precision Targeting of Origin-Specific Vascular Smooth Muscle Cells Using Intersectional Genetics.

Circulation·2025

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Published on: December 7, 2016

Chapter 9. Development of coronary vessels.

Xiu Rong Dong¹, Colin T Maguire, San-Pin Wu

¹Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.

Methods in Enzymology

|November 22, 2008

Summary

This chapter details experimental methods for studying coronary vessel development. These techniques analyze the proepicardium (PE) to mature heart vasculature, aiding research into cardiac development and remodeling.

More Related Videos

In Vitro Model of Coronary Angiogenesis

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Published on: April 15, 2020

Related Experiment Videos

Last Updated: Jun 27, 2026

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Published on: December 7, 2016

In Vitro Model of Coronary Angiogenesis

In Vitro Model of Coronary Angiogenesis

Published on: March 10, 2020

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Isolation of Endocardial and Coronary Endothelial Cells from the Ventricular Free Wall of the Rat Heart

Published on: April 15, 2020

Area of Science:

Cardiovascular Biology
Developmental Biology
Molecular Cardiology

Background:

Coronary vessel development is crucial for heart function.
Understanding the cellular and molecular mechanisms guiding this process is essential.
The proepicardium (PE) is a key source of epicardial cells involved in coronary development.

Purpose of the Study:

To summarize experimental techniques for studying coronary vessel development.
To provide foundational tools for investigating the cellular and molecular pathways involved.
To cover the process from the proepicardium to the mature coronary vascular network.

Main Methods:

Microdissection and culture of proepicardium (PE) and embryonic epicardial cells.
RNA isolation from single PE primordia and RT-PCR analysis.
Whole-mount confocal and scanning electron microscopy for imaging epicardium and vessels.
Coronary vascular corrosion casting for visualizing the entire arterial network structure.

Main Results:

Detailed descriptions of various experimental techniques are provided.
The methods allow for the study of coronary vessel development from origin to mature network.
These techniques are foundational for investigating the cellular and molecular basis of coronary development.

Conclusions:

The summarized techniques provide essential tools for studying coronary vessel development.
These methods facilitate research into the cellular and molecular pathways governing cardiac vascularization.
This chapter serves as a resource for researchers in cardiovascular and developmental biology.