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Updated: Jun 27, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
"HOOF-Print" genotyping and haplotype inference discriminates among Brucella spp. isolates from a small spatial scale
Cláudia Almendra1, Teresa Luísa Silva, Albano Beja-Pereira
1CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, Universidade do Porto, Vairão, Portugal. claudia_almendra@mail.icav.up.pt
Abstract:
We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3). We recommend that future studies use haplotype identification when analyzing multilocus population genetic data to help discriminate among microbial isolates such as Brucella.
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