Related Experiment Video
Updated: Jun 27, 2026
Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Assessing the predictive value of hyaluronan binding ability for the freezability potential of human sperm
Leah Yogev1, Sandra E Kleiman, Ron Hauser
1The Institute for the Study of Fertility, Lis Maternity Hospital, Tel Aviv Medical Center, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel. layogev@zahav.net.il
Objective:
To evaluate the predictive value of a sperm maturation test using the hyaluronan-binding assay (HBA) for freezability potential; and to determine the effect of freezing-thawing on HBA results.
Design:
Prospective study.
Setting:
Andrology laboratory at a teaching hospital.
Patient(S):
Candidates for sperm bank donation (n = 113) and active sperm bank donors (n = 16).
Intervention(S):
Semen analyses including HBA and sperm freezing-thawing.
Main Outcome Measure(S):
Percentage of sperm HBA results and other sperm parameters in relation to freezing-thawing results.
Result(S):
The predictive value of HBA for high freezability value (>or=40% postthaw motility) was significant. However, 1- and 4-hour percentage of motility had a higher predictive value for good freezability. A better prognostic value than that of HBA resutlts was also found for sperm concentration and percentage of normal morphology. Freezing-thawing had no significant influence on HBA results.
Conclusion(S):
To the best of our knowledge this is the first demonstration that sperm maturation, determined by the HBA test, has a low value for predicting freezing-thawing sperm survival.
