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DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis
Published on: September 28, 2021
Development of immobilization technique for liver microsomes
Shazia Tanvir1, Jacques Pantigny, Sandrine Morandat
1Laboratoire de Génie Enzymatique et Cellulaire, UMR 6022 CNRS, Université de Technologie de Compiègne, BP 20529-60205 Compiègne Cedex, France. tanvirsh@utc.fr
Colloids and Surfaces. B, Biointerfaces
|November 22, 2008
Summary
Researchers optimized membrane protein immobilization using physically adsorbed rat liver microsomes on solid supports. This method enhances biosensor and microreactor applications, allowing enzyme activity and support re-utilization.
Area of Science:
- Biochemistry
- Materials Science
- Biotechnology
Background:
- Membrane proteins are crucial for biological functions.
- Efficient immobilization of membrane proteins is key for biosensing and microreactor development.
- Current methods face challenges in stability and re-usability.
Purpose of the Study:
- To optimize the physical adsorption of rat liver microsomes onto solid surfaces.
- To evaluate the suitability of these immobilized membranes for biosensing and microreactor applications.
- To characterize the immobilized membrane films and assess their functional re-usability.
Main Methods:
- Physical adsorption of rat liver microsomes onto gold, mica, and macroporous aluminum oxide membranes.
- Characterization using Surface Plasmon Resonance (SPR), Atomic Force Microscopy (AFM), and Environmental Scanning Electron Microscopy (ESEM).
- Assessment of enzyme activities (Phase I and II) in the immobilized microsomal films.
Main Results:
- Successful formation of thin films of physically adsorbed rat liver microsomes on various solid supports.
- Macroporous aluminum oxide membranes demonstrated high surface area for significant membrane retention.
- Enzyme activities were retained, and the modified supports showed re-usability after washing.
Conclusions:
- Physical adsorption is an effective method for immobilizing membrane proteins for biosensing and microreactor applications.
- Macroporous aluminum oxide membranes are promising supports due to their high surface area and film-forming capabilities.
- Immobilized microsomal films offer functional re-usability, enhancing their practical utility.
