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Related Experiment Video

Updated: Jun 27, 2026

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

Niche required for inducing quiescent stem cells.

S-I Nishikawa¹, M Osawa, S Yonetani

¹Laboratory for Stem Cell Research, RIKEN Center for Developmental Biology, Kobe, Japan.

Cold Spring Harbor Symposia on Quantitative Biology

|November 22, 2008

Summary

Stem cells (SCs) enter quiescence, a dormant state crucial for their function. Research identifies fibroblast growth factor-2 (FGF-2) as a key signal in initiating this quiescent state in melanocyte stem cells.

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Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles

Isolation of Quiescent Stem Cell Populations from Individual Skeletal Muscles

Published on: December 9, 2022

Area of Science:

Stem cell biology
Dermatology
Cellular and Molecular Medicine

Background:

Quiescence is a defining characteristic of stem cells (SCs), regulated by the microenvironment or niche.
The precise molecular mechanisms governing SC quiescence remain largely unknown in most SC systems.
Melanocyte stem cells (MSCs) within the hair follicle serve as a model to study SC quiescence.

Purpose of the Study:

To identify molecular cues that induce quiescence in melanocyte stem cells (MSCs).
To develop a method for distinguishing quiescent MSCs from other cell populations based on gene expression.
To elucidate the role of specific signaling pathways in maintaining the stem cell niche.

Main Methods:

Differential gene expression profiling of melanocyte stem cells (MSCs) and other hair follicle compartments.
Development of a novel criterion for identifying MSCs based on their unique characteristics.
Experimental investigation of molecular signals, including fibroblast growth factor-2 (FGF-2), on melanoblast proliferation and quiescence.

Main Results:

A method was established to distinguish MSCs from other hair follicle cells using their intrinsic properties.
Fibroblast growth factor-2 (FGF-2) was identified as a critical signal for inducing key signatures of quiescent MSCs.
Additional signals necessary for the complete induction of the ultimate quiescent MSC state require further investigation.

Conclusions:

Fibroblast growth factor-2 (FGF-2) plays an essential role in initiating the quiescent state of melanocyte stem cells (MSCs).
The hair follicle niche provides specific cues that regulate melanocyte stem cell behavior.
Further research is needed to identify all factors contributing to the maintenance of MSC quiescence.