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Published on: July 27, 2022
Cell packing influences planar cell polarity signaling
Dali Ma1, Keith Amonlirdviman, Robin L Raffard
1Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305-5324, USA.
Summary
Planar cell polarity (PCP) propagation in Drosophila wings is influenced by cell geometry and boundary signaling. Local feedback and global cues ensure correct polarity despite tissue irregularities.
Area of Science:
- Developmental Biology
- Cell Biology
- Genetics
Background:
- Epithelial cells exhibit planar cell polarity (PCP) orthogonal to the apical-basal axis.
- A Frizzled-mediated feedback loop coordinates PCP between cells.
- The cadherin Fat orients PCP using global tissue cues.
Purpose of the Study:
- Investigate how polarity propagates in clones lacking global directional cues.
- Determine the role of cell geometry and boundary signaling in PCP.
- Understand mechanisms ensuring accurate PCP signaling in Drosophila wings.
Main Methods:
- Analysis of polarity propagation in Drosophila wing clones.
- Examination of cell geometry and nonautonomous signaling at clone boundaries.
- Investigating the interplay between global cues and local feedback in PCP.
Main Results:
- Cell geometry and nonautonomous signaling at clone boundaries dictate polarity propagation accuracy.
- Pattern elements and irregular cell geometry impede polarity propagation.
- A combination of global directional cues and local feedback overcomes geometric irregularities.
Conclusions:
- Cell geometry and boundary signaling are critical for accurate PCP propagation in clones lacking global cues.
- The Drosophila wing utilizes integrated mechanisms for robust PCP signaling.
- Understanding PCP is crucial for developmental biology and tissue organization.
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