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Updated: Jun 27, 2026
Measuring Dengue Virus RNA in the Culture Supernatant of Infected Cells by Real-time Quantitative Polymerase Chain Reaction
Published on: November 1, 2018
Effect of nitric oxide on Dengue virus replication in Aedes aegypti and Anopheles albimanus
José Ramos-Castañeda1, Cassandra González, Marco Antonio Jiménez
1Centro de Investigaciones sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Cuernavaca, México.
Unlabelled:
Dengue virus (DENV) is transmitted to humans by Aedes sp. mosquitoes. Little is known about the cellular and molecular interactions between the virus and the mosquito. The identification of resistance mechanisms could provide insight for the development of control strategies based on genetic manipulation.
Objective:
To determine the effect of nitric oxide (NO) donors/inhibitors on DENV replication in Aedes aegypti and Anopheles albimanus.
Materials And Methods:
Ae. aegypti and An. albimanus were fed with a blood suspension supplemented with DENV and donors/inhibitors of NO; DENV replication was assessed by immunofluorescence, RT-PCR and qRT-PCR parallel to NO measurement by means of the Griess reaction.
Results:
DENV replicates at 3x10(6) genome copies/day/mosquito in Aedes. In comparison, no evidence of virus genome accumulation was detected when 2 mM sodium nitroprusside, a NO donor, were added to the infected blood meal. DENV did not replicate in Anopheles unless 1 mM L-N(G)-nitroarginine methyl ester, a NO synthesis inhibitor, was added to the infected blood meal, although the absolute viral load was significantly lower than in Aedes.
Conclusions:
As in humans, NO participates in the control of the virus load in mosquitoes. However, other mechanisms could also be involved in virus resistance in Anopheles.
