Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurement of Protein Transport in Heterogeneous Environments Using Confinement k-Space Image Correlation Spectroscopy.

Biomolecules·2026
Same author

DNAi: an open-source AI tool for unbiased DNA fiber analysis.

Nucleic acids research·2026
Same author

Enhanced myelination potential of human mature oligodendrocytes by TNF and IFNG combination.

Journal of neuroinflammation·2026
Same author

MYH9 mutations differentially stabilize non-muscle myosin II filaments and induce distinct cellular aggregation phenotypes.

Cellular and molecular life sciences : CMLS·2026
Same author

Convergence and divergence of molecular mechanisms in Hebbian and homeostatic plasticity.

Frontiers in synaptic neuroscience·2026
Same author

Characterization of Dendritic Polyglycerol Amine Layers as Coatings for Improved Neural Cell Growth Surfaces.

ACS chemical neuroscience·2025

Related Experiment Video

Updated: Jun 27, 2026

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
09:49

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Published on: October 11, 2019

Patterning protein concentration using laser-assisted adsorption by photobleaching, LAPAP.

Jonathan M Bélisle1, James P Correia, Paul W Wiseman

  • 1Maisonneuve-Rosemont Hospital, University of Montreal, QC, Canada.

Lab on a Chip
|November 22, 2008
PubMed
Summary

Researchers developed a simple photobleaching technique to create precise protein patterns and gradients in vitro. This method aids in studying cellular responses in development, immunology, and cancer research.

More Related Videos

Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
12:58

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

Related Experiment Videos

Last Updated: Jun 27, 2026

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
09:49

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Published on: October 11, 2019

Control of Cell Geometry through Infrared Laser Assisted Micropatterning
11:04

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

Published on: July 10, 2021

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
12:58

Fluorescence Recovery after Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures

Published on: March 26, 2019

Area of Science:

  • Cell Biology
  • Biophysics
  • Developmental Biology

Background:

  • Studying cellular responses to molecular distribution is crucial in development, immunology, and cancer.
  • Existing methods for in vitro protein patterning lack the precision or simplicity needed for typical labs.

Purpose of the Study:

  • To present a straightforward and reliable method for generating substrate-bound protein patterns and concentration gradients in vitro.
  • To enable the study of cellular responses to in vivo-like molecular distributions.

Main Methods:

  • Utilizes photobleaching of fluorescently tagged molecules to create protein patterns.
  • Achieves sub-micron spatial resolution for precise pattern generation.
  • Employs Laser-Assisted Photobleaching (LAPAP) for generating laminin peptide gradients.

Main Results:

  • Demonstrates a simple, reproducible method for creating protein patterns and gradients.
  • Achieves high spatial resolution in generated protein distributions.
  • Successfully demonstrated axon guidance using in vitro generated laminin peptide gradients.

Conclusions:

  • The photobleaching technique offers a practical solution for in vitro protein patterning.
  • This method facilitates research in cell biology, immunology, and cancer by mimicking in vivo conditions.
  • The technique is broadly applicable for studying cellular behavior in response to defined molecular cues.