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Updated: Jun 27, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
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Published on: January 6, 2016

Non-contact protein microarray fabrication using a procedure based on liquid bridge formation.

Michael Hartmann1, Johan Sjödahl, Mårten Stjernström

  • 1School of Chemical Science and Engineering, Department of Analytical Chemistry, Royal Institute of Technology, 10044, Stockholm, Sweden.

Analytical and Bioanalytical Chemistry
|November 22, 2008
PubMed
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This study introduces a novel pressure-assisted non-contact deposition technique for protein microarray fabrication, significantly reducing common printing issues like satellite and misplaced spots for diverse, challenging samples.

Area of Science:

  • Biotechnology
  • Materials Science
  • Analytical Chemistry

Background:

  • Conventional microarrayers face challenges like satellite spots, inhomogeneous spots, misplaced spots, and sample carryover.
  • These issues limit the fabrication of high-quality protein microarrays.

Purpose of the Study:

  • To introduce a novel pressure-assisted non-contact sample deposition technique for microarray fabrication.
  • To demonstrate the technique's robustness and ability to print challenging sample solutions.

Main Methods:

  • Developed and tested a pressure-assisted non-contact deposition method.
  • Printed various difficult sample types including high-concentration proteins, cell-lysis buffer, DMSO, and polystyrene beads.
  • Utilized a fluorocarbon liquid lid to minimize evaporation and protect sensitive surfaces.

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Last Updated: Jun 27, 2026

Flow-pattern Guided Fabrication of High-density Barcode Antibody Microarray
09:05

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Published on: January 6, 2016

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
10:45

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules

Published on: June 20, 2020

High-throughput Protein Expression Generator Using a Microfluidic Platform
09:26

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

Main Results:

  • Successfully printed challenging samples that are difficult or impossible with conventional microarrayers.
  • Achieved accurate deposition of solutions causing issues like satellite spots and sample carryover.
  • Demonstrated reduced evaporation and protection of water-sensitive surfaces using the fluorocarbon lid.

Conclusions:

  • The pressure-assisted deposition technique offers a robust solution for fabricating high-quality protein microarrays.
  • This method overcomes limitations of current microarrayers, enabling precise deposition of diverse and problematic samples.
  • The fluorocarbon liquid lid enhances stability and reliability during long-term microarray printing procedures.