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Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Aerobic vaginal pathogens and their sensitivity pattern.

Shamim Mumtaz¹, Mumtaz Ahmad, Irum Aftab

¹Department of Pathology, Islamic International Medical College Trust, Rawalpindi, Pakistan. Mumtaz_dr@hotmail.com

Journal of Ayub Medical College, Abbottabad : JAMC

|November 26, 2008

Summary

This study investigated aerobic vaginal pathogens and antibiotic sensitivity, finding Staphylococcus aureus most common. Effective treatments like carbapenems are expensive, necessitating a new antimicrobial policy.

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Area of Science:

Microbiology
Gynecology

Background:

Vaginal flora is complex, with infections causing discharge.
Intermediate flora remains undiagnosed in 7-72% of vaginitis cases.
Understanding aerobic vaginal isolates is crucial, especially in pregnant women at risk of preterm delivery.

Purpose of the Study:

To identify aerobic vaginal isolates.
To determine their culture characteristics.
To assess antibiotic sensitivity of these isolates.

Main Methods:

Collected 1,923 high vaginal swabs from indoor and outdoor patients.
Performed cultures and antibiotic susceptibility testing.
Analyzed data over a two-year period (April 2004-March 2006).

Main Results:

Significant microbial growth found in 731 samples.
Staphylococcus aureus was the predominant pathogen, particularly in women aged 31-50.
Few conventional antibiotics showed high sensitivity; carbapenems and beta-lactam/beta-lactamase inhibitor combinations were most effective.

Conclusions:

Thorough investigation of gynecological infections, including cultures, is essential.
Currently effective antibiotics are expensive, highlighting the need for an antimicrobial policy.
Reserve potent antimicrobial agents for severe, life-threatening infections.