Related Experiment Video
Updated: Jun 27, 2026
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
Published on: August 25, 2022
[Study on JAKs-STATs signal transduction in neonatal rats with PVL]
Xi-ge Wang1, Ying Xiong, Wen-jin Guo
1Department of Pediatrics, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To examine the changes of JAKs-STATs pathway in the subventricular zone and choroid plexus of neonatal rats with PVL.
Methods:
A PVL model was established by right common carotid artery ligation followed by 4 h 6% oxygen exposure in 2-day-rat, the neonatal rats performed a sham operation, without hypoxia-ischemia were used as the control grobp. The rats were sacrificed at 0 h, 3 h, 6 h, 12 h, 1 d, 3 d. 7 d of HI, and the brain tissues were collected, immunohistochemistry was applied to detect the expression of P-JAK2 and P-STAT3.
Results:
The expression levels of P-JAK2 and P-STAT3 increased significantly after HI, peaked at 1 d, and remained at a higher level than control until 7 days of HI, the difference was significant (P < 0.01).
Conclusion:
HI resulted in the activation of JAKs-STATs pathway in the subventricular zone and choroid plexus, and this pathway might participated in the pathophysiological process of PVL.
