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Updated: Jun 27, 2026
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
Published on: October 19, 2013
[Expression pattern of polycomb gene Nspc1 at the early developmental stage in zebrafish]
Xu-Dong Wu1, Min Zhang, Yan-Hua Gong
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Objective:
To study the expression pattern of Polycomb gene Nspc1 at the early developmental stage in zebrafish.
Methods:
In situ hybridization probe for Nspc1 was designed according to the GenBank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right time.
Results:
Nspc1 was expressed globally at the early stage. Its expression specificity began at the somites stage, mainly in the nervous system of the head.
Conclusion:
Nspc1 may play essential roles in the early stage development of zebrafish, especially in the nervous system.
