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Updated: Jun 27, 2026

OnePot PURE Cell-Free System
Published on: June 23, 2021
[Three-step purification of preparative-scale antiCD20 (Fab')2]
Jin-Hong Wang1, Ming Yang, Dong-Mei Fan
1State Key Laboratory of Experimental Hematology, Institute of Hematology, CAMS and PUMC, Tianjin 300020, China.
Objective:
To establish a three-step purification method of preparative-scale antiCD20 (Fab')2 using AKTA prime.
Methods:
AntiCD20 (Fab')2 was extracted by hyperosmotic solution and then purified by CM sepharose FF, phenyl sepharose FF, and protein G sepharose FF.
Results:
Around 8 mg anti-CD20 (Fab')2, whose purification was 96.678%, was purified. The antigen-binding activity of antiCD20 (Fab')2 was similar to that of antiCD20 (Fab')2 purified by protein G sepharose FF and S-100.
Conclusion:
The three-step purification method can obtain high-purity preparative-scale antiCD20 (Fab')2 in a simple way.
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