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Updated: Jun 27, 2026
Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
Published on: February 8, 2018
Immunohistochemical expression of multidrug resistance proteins in mature T/NK-cell lymphomas
Arzu Saglam1, Mutlu Hayran, Aysegul H Uner
1Department of Pathology, Oncology Institute, Hacettepe University Faculty of Medicine, Ankara, Turkey.
Abstract:
Multidrug resistance (MDR) is defined as resistance of tumor cells to a wide spectrum of structurally and functionally unrelated drugs. One of the most important mechanisms in mediating MDR is that involving cellular drug efflux transporters. Drug resistance is a common and formidable obstacle to therapy in mature T/NK-cell lymphomas and the MDR phenotype is thought to be one of the contributing mechanisms. In this study we assessed the immunohistochemical expression of P-gp (P-glycoprotein), MRP-1 (multidrug resistance associated protein 1), BCRP (breast cancer resistance protein) and LRP (lung resistance protein) in 45 mature T/NK-cell lymphomas diagnosed at our hospital. We detected P-gp expression in 31% (13/42), MRP-1 expression in 74% (31/42), BCRP in 78% (32/41) and LRP in 59% (26/44) of the cases. These findings show that our T/NK-cell lymphoma cases display high frequency of MDR protein expression.
Insights
Mature T/NK-cell lymphomas frequently exhibit multidrug resistance (MDR) proteins. This study found high expression of P-glycoprotein, MRP-1, BCRP, and LRP, contributing to treatment challenges.
Area of Science:
- Oncology
- Molecular Biology
- Hematology
Background:
- Multidrug resistance (MDR) poses a significant challenge in treating mature T/NK-cell lymphomas.
- Cellular drug efflux transporters are key mediators of the MDR phenotype.
- Understanding MDR mechanisms is crucial for improving therapeutic outcomes.
Purpose of the Study:
- To investigate the expression of major drug efflux transporters in mature T/NK-cell lymphomas.
- To assess the frequency of P-glycoprotein (P-gp), multidrug resistance associated protein 1 (MRP-1), breast cancer resistance protein (BCRP), and lung resistance protein (LRP) expression.
Main Methods:
- Immunohistochemical analysis was performed on 45 cases of mature T/NK-cell lymphomas.
- Expression levels of P-gp, MRP-1, BCRP, and LRP were quantified.
Main Results:
- High frequencies of MDR protein expression were observed: MRP-1 in 74%, BCRP in 78%, LRP in 59%, and P-gp in 31% of cases.
- The study identified a significant prevalence of these resistance markers in the studied lymphoma cohort.
Conclusions:
- Mature T/NK-cell lymphomas frequently express multiple drug resistance proteins.
- The high expression of P-gp, MRP-1, BCRP, and LRP suggests their involvement in therapeutic resistance in these lymphomas.
- These findings highlight potential targets for overcoming MDR in T/NK-cell lymphomas.
