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Analysis of a Corynebacterium glutamicum hom gene coding for a feedback-resistant homoserine dehydrogenase
D J Reinscheid1, B J Eikmanns, H Sahm
1Institut für Biotechnologie, 1 des Forschungszentrums Jülich GmbH, Federal Republic of Germany.
Journal of Bacteriology
|May 1, 1991
Abstract:
From a Corynebacterium glutamicum mutant possessing a homoserine dehydrogenase resistant to feedback inhibition by L-threonine, the corresponding gene (homFBR) was analyzed and compared with the wild-type hom gene. DNA fragment exchange experiments between both genes showed that a 0.23-kb region close to the 3' terminus of homFBR was responsible for deregulation. Nucleotide sequence analysis revealed a single transition from G to A in homFBR leading to replacement of glycine-378 by glutamate in the mutant homoserine dehydrogenase.