Related Experiment Video
Updated: Jun 27, 2026
Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells
Published on: July 11, 2025
Mouse bone marrow mesenchymal stem cells acquire CD45-CD106+ immunophenotype only at later passages
Y Y Ooi1, R Ramasamy, S Vidyadaran
1Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra, Malaysia.
Abstract:
Classically, MSC are identified by a CD45-CD106+ phenotype. In this study, we found that mouse MSC achieve this characteristic phenotype only at later passages. With increasing passages, CD45 (hematopoietic marker) expression shifts to negativity, whereas CD106 (vascular cell adhesion molecule-1) expression becomes increasingly positive. These results demonstrate that MSC cells cultured from mouse bone marrow acquire a classical MSC immunophenotype (CD45-CD106+) in later passages.
Insights
Mouse mesenchymal stem cells (MSCs) acquire their classical CD45-CD106+ immunophenotype at later passages. This study tracked CD45 and CD106 expression changes during MSC culture.
Area of Science:
- Cell Biology
- Immunology
- Stem Cell Research
Background:
- Mesenchymal stem cells (MSCs) are traditionally identified by a CD45-CD106+ immunophenotype.
- Understanding MSC immunophenotype development is crucial for their therapeutic applications.
Purpose of the Study:
- To investigate the temporal changes in MSC immunophenotype during in vitro culture.
- To determine when mouse MSCs achieve the classical CD45-CD106+ phenotype.
Main Methods:
- Culturing mouse bone marrow-derived MSCs.
- Analyzing cell surface marker expression (CD45 and CD106) across multiple passages using flow cytometry.
Main Results:
- CD45 expression (hematopoietic marker) decreased over passages, becoming negative.
- CD106 expression (vascular cell adhesion molecule-1) increased with successive passages.
- The classical CD45-CD106+ MSC immunophenotype was observed in later culture passages.
Conclusions:
- Mouse MSCs attain their characteristic CD45-CD106+ phenotype progressively during in vitro expansion.
- Passage number is a critical factor influencing the immunophenotypic identification of MSCs.
