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Updated: Jun 27, 2026
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Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells
Published on: July 11, 2025
Mouse bone marrow mesenchymal stem cells acquire CD45-CD106+ immunophenotype only at later passages
Y Y Ooi1, R Ramasamy, S Vidyadaran
1Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra, Malaysia.
The Medical Journal of Malaysia
|November 26, 2008
Summary
Mouse mesenchymal stem cells (MSCs) acquire their classical CD45-CD106+ immunophenotype at later passages. This study tracked CD45 and CD106 expression changes during MSC culture.
Area of Science:
- Cell Biology
- Immunology
- Stem Cell Research
Background:
- Mesenchymal stem cells (MSCs) are traditionally identified by a CD45-CD106+ immunophenotype.
- Understanding MSC immunophenotype development is crucial for their therapeutic applications.
Purpose of the Study:
- To investigate the temporal changes in MSC immunophenotype during in vitro culture.
- To determine when mouse MSCs achieve the classical CD45-CD106+ phenotype.
Main Methods:
- Culturing mouse bone marrow-derived MSCs.
- Analyzing cell surface marker expression (CD45 and CD106) across multiple passages using flow cytometry.
Main Results:
- CD45 expression (hematopoietic marker) decreased over passages, becoming negative.
- CD106 expression (vascular cell adhesion molecule-1) increased with successive passages.
- The classical CD45-CD106+ MSC immunophenotype was observed in later culture passages.
Conclusions:
- Mouse MSCs attain their characteristic CD45-CD106+ phenotype progressively during in vitro expansion.
- Passage number is a critical factor influencing the immunophenotypic identification of MSCs.
