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Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
Positive Regulator Molecules02:39

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

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Related Experiment Video

Updated: Jun 27, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Cdc20, an activator at last.

Jennifer A Benanti1, David P Toczyski

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94115, USA.

Molecular Cell
|November 26, 2008
PubMed
Summary

Cdc20 plays a dual role in cell division, aiding in substrate recruitment and activation of the anaphase-promoting complex. This discovery deepens our understanding of cell cycle regulation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The anaphase-promoting complex (APC/C) is a crucial ubiquitin ligase complex that regulates cell cycle progression.
  • APC/C activity is tightly controlled by regulatory proteins, including Cdc20 and Cdh1.
  • Cdc20 is known to facilitate the recruitment of APC/C substrates, such as securin and M-phase cyclins.

Purpose of the Study:

  • To investigate the precise role of Cdc20 in the regulation of the anaphase-promoting complex (APC/C).
  • To determine if Cdc20 has functions beyond substrate recruitment for APC/C.
  • To elucidate the mechanisms by which Cdc20 influences APC/C activity.

Main Methods:

  • Biochemical assays to study APC/C complex formation and activity.
  • In vitro reconstitution experiments using purified proteins.

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Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
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Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Published on: March 2, 2018

Related Experiment Videos

Last Updated: Jun 27, 2026

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome
11:15

Pooled shRNA Screen for Reactivation of MeCP2 on the Inactive X Chromosome

Published on: March 2, 2018

  • Analysis of substrate ubiquitination in the presence and absence of Cdc20.

    • Main Results:

    • Kimata et al. demonstrate that Cdc20 is essential for the activation of the anaphase-promoting complex.
    • Cdc20 directly promotes the catalytic activity of the APC/C, in addition to its role in substrate targeting.
    • The study provides evidence for a novel mechanism of APC/C regulation mediated by Cdc20.

    Conclusions:

    • Cdc20 functions as a dual-action regulator of the anaphase-promoting complex.
    • This dual role of Cdc20 in substrate recruitment and catalytic activation is critical for proper cell cycle progression.
    • The findings expand the understanding of APC/C regulation and its importance in cell division.