Related Experiment Video
Updated: Jun 27, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
In vitro nicotine induced superoxide mediated DNA fragmentation in lymphocytes: protective role of Andrographis
Subhasis Das1, Sreeparna Neogy, N Gautam
1Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore 721 102, West Bengal, India.
Abstract:
Nicotine is a pharmacologically active substance and potent recreational drug present in smoke and smokeless tobacco products. The present study was initiated to investigate the protective role of Andrographis paniculata products (andrographolide and aqueous extract) on in vitro nicotine induced lymphocyte toxicity. Andrographolide and aqueous extract was isolated and characterized by HPLC, FTIR, TLC and biochemical assays. Significant (P<0.05) increase of superoxide anion generation, lipid peroxidation, protein oxidation and DNA fragmentation and decrease of cell viability, SOD and GSH content were observed in both 10mM and 100mM nicotine exposure. Different concentration of andrographolide and aqueous extract from A. paniculata supplement decreased oxidative stress in lymphocytes with the fall in superoxide anion generation, lipid peroxidation, protein oxidation, DNA fragmentation and rise in cell viability and the activities of the antioxidant enzymes; SOD and GSH. The above findings indicate that A. paniculata products modulate the nicotine induced toxicity in lymphocytes through decreased superoxide mediated oxidative stress and DNA fragmentation. Hence, A. paniculata can be used as therapeutic means against nicotine mediated lymphocytes function.
More Related Videos
09:25Methods to Evaluate Cytotoxicity and Immunosuppression of Combustible Tobacco Product Preparations
Published on: January 10, 2015
09:30A Microcontroller Operated Device for the Generation of Liquid Extracts from Conventional Cigarette Smoke and Electronic Cigarette Aerosol
Published on: January 18, 2018
Related Concept Videos
Drugs Acting on Autonomic Ganglia: Stimulants
Ganglionic stimulants activate NM nicotinic receptors in autonomic ganglia, falling into two categories: nicotine mimetics [e.g., lobeline, dimethylpiperazine, tetramethylammonium] and muscarinic receptor agonists [e.g., muscarine, methacholine]. The first category's action is rapid and blocked by nicotinic receptor antagonists, while the second category's action is delayed and blocked by atropine-like agents. Nicotine, an alkaloid, affects the heart rate by stimulating sympathetic or...
Mutagenicity and Carcinogenicity