Related Experiment Video
Updated: Jun 27, 2026
Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
Published on: February 28, 2019
Does storage time influence postthaw survival and pregnancy outcome? An analysis of 11,768 cryopreserved human
Ryan Riggs1, Jacob Mayer, Donna Dowling-Lacey
1Department of Obstetrics and Gynecology, The Jones Institute for Reproductive Medicine, Eastern Virginia Medical School, Norfolk, Virginia 23507, USA.
Objective:
To evaluate the impact of cryopreservation storage duration on embryo survival, implantation competence, and pregnancy outcome.
Design:
Retrospective study.
Setting:
Academic tertiary-referral infertility center.
Patient(S):
In vitro fertilization patients and recipients of oocyte donation cycles who had cryopreserved embryos and underwent at least one thaw cycle from 1986 to 2007.
Intervention(S):
None.
Main Outcome Measure(S):
Postthaw survival proportion and implantation, clinical pregnancy, miscarriage, and live birth rates.
Result(S):
Length of storage time did not have a significant effect on postthaw survival for IVF or oocyte donation cycles, or for embryos frozen at the pronuclear or cleavage stages. There was no significant impact of the duration of storage on clinical pregnancy, miscarriage, implantation, or live birth rate, whether from IVF or oocyte donation cycles. Logistic regression analysis demonstrated that the length of storage time or developmental stage at freezing were not predictive of embryo survival or pregnancy outcome. Only oocyte age, survival proportion, and number of transferred embryos were positive predictors of pregnancy outcome.
Conclusion(S):
Cryostorage duration did not adversely affect postthaw survival or pregnancy outcome in IVF or oocyte donation patients.
