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Related Experiment Videos

Complement subcomponent C1q stimulates Ig production by human B lymphocytes.

K R Young1, J L Ambrus, A Malbran

  • 1Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892.

Journal of Immunology (Baltimore, Md. : 1950)
|May 25, 1991
PubMed
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The complement protein C1q stimulates immunoglobulin (Ig) production in human B cells, independent of proliferation. C1q also influences Ig isotype selection in B cells from patients with systemic lupus erythematosus (SLE).

Area of Science:

  • Immunology
  • Complement System
  • B cell Biology

Background:

  • Immunoglobulin (Ig) production by human B lymphocytes is regulated by complex cellular and soluble factor interactions.
  • Components of the complement system, such as C3a, C3b, C3d, and C5a, are known to modulate B cell processes.
  • Systemic lupus erythematosus (SLE) is characterized by polyclonal B lymphocyte activation.

Purpose of the Study:

  • To investigate the effect of the complement subcomponent C1q on human B lymphocyte immunoglobulin production.
  • To determine if C1q influences Ig isotype selection in B cells from patients with SLE.

Main Methods:

  • C1q was added to Staphylococcus aureus Cowan-activated tonsillar B lymphocytes and the human B cell line SKW 6.4.
  • Immunoglobulin production, proliferation, and mRNA levels for the mu Ig H chain were assessed.

Related Experiment Videos

  • The effect of C1q on Ig secretion from B cells of SLE patients was analyzed.

    • Main Results:

    • C1q binds to resting and activated B cells and stimulates immunoglobulin production without enhancing proliferation.
    • C1q-induced Ig production correlates with increased mRNA levels for the mu Ig H chain.
    • In SLE patients, C1q selectively stimulated the production of the non-predominant Ig isotype (IgG vs. IgM).

    Conclusions:

    • C1q directly interacts with human B lymphocytes to stimulate immunoglobulin production.
    • C1q influences Ig isotype switching in B cells, distinct from B cell differentiation factors.
    • C1q may play a significant role in regulating Ig production in both healthy individuals and those with B cell abnormalities like SLE.