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Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
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Dpb11 activates the Mec1-Ddc2 complex.

Daniel A Mordes1, Edward A Nam, David Cortez

  • 1Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.

Proceedings of the National Academy of Sciences of the United States of America
|November 26, 2008
PubMed
Summary
This summary is machine-generated.

Researchers found that Saccharomyces cerevisiae Dpb11 protein interacts with Mec1-Ddc2 kinase complex, stimulating its activity. This interaction and activation mechanism are conserved in humans, highlighting Dpb11 as a TopBP1 ortholog.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • The Saccharomyces cerevisiae Mec1-Ddc2 complex is crucial for maintaining genomic integrity.
  • The Dpb11 protein is involved in DNA replication and cell cycle checkpoints.

Purpose of the Study:

  • To investigate the interaction between Dpb11 and the Mec1-Ddc2 complex.
  • To elucidate the functional role of Dpb11 in regulating Mec1-Ddc2 kinase activity.
  • To determine if this interaction is conserved in humans.

Main Methods:

  • Genetic interaction studies.
  • Physical interaction assays.
  • In vitro kinase assays.

Main Results:

  • A genetic and physical interaction between Dpb11 and Mec1-Ddc2 was identified.
  • A C-terminal domain of Dpb11 associates with Mec1-Ddc2 and stimulates its kinase activity in a Ddc2-dependent manner.
  • Mec1 phosphorylates Dpb11, amplifying the stimulation of Mec1-Ddc2 kinase activity.

Conclusions:

  • Dpb11 is a functional ortholog of human TopBP1.
  • The mechanism of Mec1/ATR activation is conserved between yeast and humans.
  • This study reveals a conserved regulatory pathway for genomic integrity.