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Control of doublesex alternative splicing by transformer and transformer-2 in Drosophila
K Hoshijima1, K Inoue, I Higuchi
1Department of Biophysics, Faculty of Science, Kyoto University, Japan.
Science (New York, N.Y.)
|May 10, 1991
Abstract:
Sex-specific alternative processing of doublesex (dsx) precursor messenger RNA (pre-mRNA) regulates somatic sexual differentiation in Drosophila melanogaster. Cotransfection analyses in which the dsx gene and the female-specific transformer (tra) and transformer-2 (tra-2) complementary DNAs were expressed in Drosophila Kc cells revealed that female-specific splicing of the dsx transcript was positively regulated by the products of the tra and tra-2 genes. Furthermore, analyses of mutant constructs of dsx showed that a portion of the female-specific exon sequence was required for regulation of dsx pre-messenger RNA splicing.