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Updated: Jun 27, 2026

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
Published on: April 20, 2016
Origin, homeostasis and function of Langerhans cells and other langerin-expressing dendritic cells
Miriam Merad1, Florent Ginhoux, Matthew Collin
1Department of Gene and Cell Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA. Miriam.Merad@mssm.edu
Abstract:
Langerhans cells (LCs) are a specialized subset of dendritic cells (DCs) that populate the epidermal layer of the skin. Langerin is a lectin that serves as a valuable marker for LCs in mice and humans. In recent years, new mouse models have led to the identification of other langerin(+) DC subsets that are not present in the epidermis, including a subset of DCs that is found in most non-lymphoid tissues. In this Review we describe new developments in the understanding of the biology of LCs and other langerin(+) DCs and discuss the challenges that remain in identifying the role of different DC subsets in tissue immunity.
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