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Updated: Jun 27, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Simultaneous dual-band ultra-high resolution full-field optical coherence tomography
Delphine Sacchet1, Julien Moreau, Patrick Georges
1Laboratoire Charles Fabry de l'Institut d'Optique, CNRS UMR 8501, Université Paris-Sud, Campus Polytechnique, RD128, 91127 Palaiseau Cedex, France. delphine.sacchet@institutoptique.fr
Abstract:
Ultrahigh-resolution full-field optical coherence tomography (FF-OCT) is demonstrated in the 800 nm and 1200 nm wavelength regions simultaneously using a Silicon-based (Si) CCD camera and an Indium Gallium Arsenide (InGaAs) camera as area detectors and a halogen lamp as illumination source. The FF-OCT setup is optimized to support the two broad spectral bands in parallel, achieving a detection sensitivity of approximately 90 dB and a micrometer-scale resolution in the three directions. Images of ex vivo biological tissues are presented (rabbit trachea and Xenopus laevis tadpole) with an increase in penetration depth at 1200 nm. A color image representation is applied to fuse both images and enhance spectroscopic property visualization.
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