Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial Expression of Concern: Adenomatous polyposis coli (APC) regulates miR17-92 cluster through β-catenin pathway in colorectal cancer.

Oncogene·2024
Same author

Comparison of traditional two-injection dorsal digital block versus transthecal and subcutaneous single-injection digital block: A systematic review and meta-analysis.

Hand surgery & rehabilitation·2021
Same author

Phylogenetic and phylodynamic analyses of hepatitis C virus subtype 1a in Okinawa, Japan.

Journal of viral hepatitis·2018
Same author

Management and outcomes following pancreaticoduodenectomy for ampullary adenocarcinoma.

American journal of surgery·2017
Same author

Synchronous rectal adenocarcinoma and splenic marginal zone lymphoma.

Current oncology (Toronto, Ont.)·2016
Same author

A stem cell niche dominance theorem.

Journal of stem cells & regenerative medicine·2014
Same journal

Tumor Invasive Border Index (TIBI) in colorectal cancer: linking infiltrative morphology to molecular insights.

The Journal of pathology·2026
Same journal

Lipodystrophy and adipose tissue recovery are mediated by the Wnt/lipogenesis axis during skin fibrosis.

The Journal of pathology·2026
Same journal

Optical mapping reveals a higher level of large-scale structural variants in a family with paternally transmitted myotonic dystrophy and independent Parkinson's disease.

The Journal of pathology·2026
Same journal

FTO-mediated m6A modification of protein disulfide-isomerase activates VEGFA-VEGFR2 to suppress programmed cell death in osteosarcoma.

The Journal of pathology·2026
Same journal

Dopamine inhibits retinal pathological neovascularization in the oxygen-induced retinopathy mouse model.

The Journal of pathology·2026
Same journal

Aberrant alternative splicing of purinergic receptor P2RX4 prevents sensitivity towards combinatorial treatment in colorectal and pancreatic cancer.

The Journal of pathology·2026
See all related articles

Related Experiment Video

Updated: Jun 27, 2026

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation
11:38

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

Published on: October 4, 2024

Inferring human stem cell behaviour from epigenetic drift.

D Shibata1

  • 1University of Southern California Keck School of Medicine, Department of Pathology, Los Angeles, CA 90033, USA. dshibata@hsc.usc.edu

The Journal of Pathology
|November 26, 2008
PubMed
Summary
This summary is machine-generated.

Human stem cell fates may be inferred by analyzing genomic DNA methylation patterns, which accumulate with cell divisions like a molecular clock. This epigenetic drift reflects stem cell behavior and lineage history.

More Related Videos

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

Related Experiment Videos

Last Updated: Jun 27, 2026

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation
11:38

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

Published on: October 4, 2024

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

Area of Science:

  • Genomics
  • Epigenetics
  • Developmental Biology

Background:

  • Inferring human stem cell fates is challenging with conventional methods.
  • Genomes are replicated with high fidelity, but errors accumulate over time.
  • DNA methylation patterns are copied during replication and can drift with age.

Purpose of the Study:

  • To explore the potential of using genomic information to infer human stem cell fates.
  • To investigate DNA methylation patterns as 'epigenetic' clocks for stem cell lineages.
  • To demonstrate how methylation patterns can encode stem cell ancestries.

Main Methods:

  • Analyzing DNA methylation patterns in specific CpG sites within mitotic tissues.
  • Applying the 'molecular clock hypothesis' to accumulated replication errors.
  • Examining stem cell genealogies in colon crypt niches and hair follicles.

Main Results:

  • DNA methylation patterns measurably drift with age in certain tissues, acting as epigenetic clocks.
  • Replication errors and methylation drift accumulate in long-lived stem cell lineages.
  • Distinct genealogical patterns were observed in colon crypts and hair follicles.

Conclusions:

  • Genomic analysis, particularly DNA methylation patterns, can potentially reveal human stem cell histories.
  • Epigenetic drift serves as a record of stem cell behavior and lineage.
  • The genealogy of any human cell may be inferred by 'reading' its genome.