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[Expression level changes of inhibitor of differentiation 1 during ATRA-induced acute promyelocytic leukemia cells
Pei-zheng Zheng1, Chun-jun Zhao, Hui-yong Fan
1Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Objective:
To study the role of inhibitor of differentiation 1 (ID1) in ATRA-induced acute promyelocytic leukemia (APL) cells differentiation.
Methods:
The expression of ID1 was detected by cDNA microarray, cycloheximide inhibition test, real-time RT-PCR and western blot.
Results:
The expression of ID1 gene was up-regulated in ATRA-induced NB4 cells and APL cells from two patients and was independent on other proteins synthesis. ID1 expression level reached the peak at 2 h in NB4 cells induced by ATRA, its relative expression level was (359.4 +/- 48.7)-fold greater than control. ID1 expression level reached the peak at 2 h in bone marrow cells from APL patents treated with ATRA, and its level detected 3 times in one of the patient was (311.1 +/- 48.7) fold of control. The expression of ID1 protein was not up-regulated in ATRA resistant NB4-R2 cells after ATRA treatment.
Conclusion:
ID1 may be involved in ATRA-induced granulocytic differentiation as an ATRA-targeted gene.
