Related Experiment Video
Updated: Jun 27, 2026
Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro
Published on: April 15, 2022
[Degradation of electrodeposited calcium phosphate and calcium phosphate-chitosan coatings in vitro]
Li-Na Wang1, Yi-Ning Wang, Jia-Wei Wang
1Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Objective:
To investigate the degradation of electrodeposited calcium phosphate (ECP) coating and calcium phosphate/chitosan (ECPC) coating in vitro.
Methods:
Osteoclasts were isolated from neonatal rabbit long bone cavities and incubated with ECP and ECPC coatings. Calcium ion concentrations in the culture medium were analyzed at 3 days and 6 days. The osteoclastic resorption was observed with scanning electron microscope.
Results:
Both coatings demonstrated osteoclastic resorption lacunae. The calcium ion concentrations of the culture mediums were decreased when incubated with calcium phosphate coatings (P < 0.05). Compared with coatings cultured with osteoclasts, the calcium ion concentrations of those cultured without osteoclasts were higher on day 3 (P > 0.05) but lower on day 6 (P < 0.05).
Conclusions:
Both ECP and ECPC coatings can be resorbed by osteoclasts in vitro and can dissolve in the culture medium.
