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Related Experiment Video

Updated: Jun 27, 2026

Murine Drinking Models in the Development of Pharmacotherapies for Alcoholism: Drinking in the Dark and Two-bottle Choice
07:31

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Published on: January 7, 2019

A comparative study of two methods for attaining constant alcohol levels.

Remco W M Zoethout1, Joop M A van Gerven, Glenn J H Dumont

  • 1Centre for Human Drug Research, Department of Anaesthesiology, Leiden, the Netherlands. rzoethout@chdr.nl

British Journal of Clinical Pharmacology
|November 27, 2008
PubMed
Summary

The alcohol clamping method achieves more accurate and stable blood alcohol levels compared to the prekinetic procedure. This method is valuable for future alcohol interaction and pharmacodynamic studies.

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Area of Science:

  • Pharmacology
  • Pharmacokinetics
  • Drug Interactions

Background:

  • Studying alcohol effects or drug-alcohol interactions requires constant blood alcohol levels.
  • Existing methods for achieving pseudo-steady state alcohol levels often result in variable individual concentration profiles.
  • Accurate control of alcohol concentration is crucial for reliable research outcomes.

Purpose of the Study:

  • To compare the accuracy and reliability of two alcohol administration methods: a 'two-step prekinetic procedure' and a 'clamping method'.
  • To evaluate the effectiveness of a novel breath alcohol concentration (BrAC)-guided clamping paradigm for maintaining stable alcohol levels.

Main Methods:

  • The prekinetic procedure involved determining individual pharmacokinetic (PK) parameters and calculating infusion regimens for a target BrAC of 0.65 g/l.
  • The clamping procedure utilized a spreadsheet-based paradigm with BrAC-guided adjustments to maintain a stable BrAC of 0.6 g/l.
  • Both methods were applied on separate occasions to compare their performance in controlling alcohol concentration.

Main Results:

  • The clamping method achieved a mean BrAC of 0.61 g/l, which did not significantly differ from the target (1.0% deviation).
  • The prekinetic procedure resulted in a mean BrAC of 0.59 g/l, deviating significantly from the target by 9.7%.
  • The clamping method demonstrated significantly less inter-subject variation (CV 6.2%) compared to the prekinetic procedure (CV 14.6%).

Conclusions:

  • The alcohol clamping method provides more accurate alcohol concentration control than the prekinetic procedure based on PK modeling.
  • The clamping method does not necessitate an additional prestudy occasion for parameter determination.
  • The developed alcohol clamping paradigm is a valuable tool for future research on alcohol interactions and acute alcohol pharmacodynamics.