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Updated: Jun 27, 2026

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
Published on: August 9, 2019
Changing modified regions in the genome in hematopoietic stem cell differentiation
Tsuyoshi Hamada1, Satoshi Murasawa, Ayumi Yokoyama
1Department of Anatomy and Neurobiology, National Defense Medical Collage, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Researchers identified key DNA methylation changes during hematopoietic stem cell (CD133+ cell) differentiation using a novel method. This advance aids in understanding epigenetic regulation in stem cell development.
Area of Science:
- Epigenetics
- Stem Cell Biology
- Genomics
Background:
- Hematopoietic stem cell (CD133+ cell) differentiation involves significant gene expression changes regulated by epigenetic mechanisms.
- Analyzing these epigenetic modifications is crucial for understanding stem cell development.
- Obtaining sufficient uniform CD133+ cells for genomic analysis presents a significant challenge.
Purpose of the Study:
- To identify genomic DNA regions with altered epigenetic modifications during human CD133+ cell differentiation.
- To overcome the limitation of small cell numbers in epigenetic analysis.
Main Methods:
- Differential Methylation Site Scanning (DMSS) was employed to detect differentially methylated regions in the genome.
- This method is suitable for analyzing epigenetic changes in limited cell populations.
Main Results:
- DMSS successfully identified genomic DNA regions with changing methylation patterns during CD133+ cell differentiation.
- Three specific DNA fragments were cloned, corresponding to genes involved in centrosomal function (Cep68), cytoskeletal organization (TRIOBP), and metabolic regulation (AMPKb).
Conclusions:
- The study successfully applied DMSS to identify epigenetic modifications during stem cell differentiation from limited cell samples.
- The identified DNA fragments represent novel targets for further investigation into the epigenetic regulation of CD133+ cell differentiation.
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