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[Caudal-related homeobox 2 gene expression and its effects on human gastric carcinoma cell line]
Lei Li1, Qiang Xiao, Yu-bo Xie
1Department of Surgery, the First Hospital Affiliated to Guangxi Medical University, Nanning 530021, China.
Objective:
To investigate the effect of human caudal-related homeobox 2 (Cdx2) gene expression on human gastric carcinoma cell line MGC-803.
Methods:
pCMV-Cdx2-HA eukaryotic expression plasmid was constructed by using DNA recombinant method. The MGC-803 cells were divided into 4 groups: non-transfected, transfected with pCMV-HA, transfected with pCMV-GAPDH-HA, transfected with pCMV-Cdx2-HA. Cdx2 gene and its protein expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot techniques respectively. The effects of Cdx2 overexpression on the growth of MGC-803 cells in vitro was assessed by measuring MTT, flow cytometry and transmission electron microscopy analysis.
Results:
In MGC-803 cells transfected with pCMV-Cdx2-HA, cell proliferation was significantly suppressed, the cells was ultrastructurally destroyed, cell cycle progression was blocked in G0/G1 and apoptosis rate was higher (P<0.05) in comparison with non-transfected cells or cells transfected with empty vector.
Conclusion:
It indicated that Cdx2 gene can suppress the growth of gastric carcinoma and may save as a novel therapeutic target.
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