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Updated: Jun 27, 2026
High-Efficiency Transduction of Liver Cancer Cells by Recombinant Adeno-Associated Virus Serotype 3 Vectors
Published on: March 22, 2011
Immunogene therapy by adenovirus vector expressing CD40 ligand for metastatic liver cancer in rats
Ken Hanyu1, Tomonori Iida, Hiroaki Shiba
1Department of Surgery, Institute of DNA Medicine, The Jikei University School of Medicine, Tokyo, Japan.
Background:
We have explored a gene-therapeutic approach to stimulate antitumor immunity by adenoviral-mediated transfer of CD40 ligand (CD40L) to treat metastatic liver cancer in a rat model.
Materials And Methods:
Rat metastatic liver cancer cells were implanted into the back of rats bilaterally. When the larger tumor reached 8.0 mm in diameter, adenovirus vector-expressing mouse CD40L was injected intratumorally as treatment group (n=5), while LacZ was injected in the control group (n=5).
Results:
In the control group, the tumor gradually grew to be 20.7+/-1.6 (mean+/-SD) mm in intratumorally injected tumors and 21.8+/-3.7 mm in opposite tumors seven weeks after injection, respectively. In contrast, in the treatment group, the tumor was reduced to 3.6+/-8.2 mm and 3.7+/-8.2 mm. The tumor growth and survival rate were significantly different (p<0.001).
Conclusion:
Adenovirus vector-mediated CD40L gene therapy is an effective therapeutic method for metastatic liver cancer.
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