Related Experiment Video

Updated: Jun 27, 2026

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors
10:44

Translating Extracellular Electron Transfer Activities with Organic Electrochemical Transistors

Published on: January 31, 2025

Factors acting on Mos1 transposition efficiency

Ludivine Sinzelle1, Gwenhael Jégot, Benjamin Brillet

  • 1Université François Rabelais de Tours, GICC, UFR des Sciences & Techniques, Parc Grandmont, 37200 Tours, France. sinzellel@yahoo.fr

BMC Molecular Biology
|November 28, 2008
PubMed
Abstract

Insights

Mariner-like elements (MLEs) transposition efficiency is influenced by DNA sequence, length, and super-helicity. Experimental conditions significantly impact transposition outcomes, with auto-integration limiting efficiency in vitro.

Area of Science:

  • Genomics
  • Molecular Biology
  • Transposable Elements

Background:

  • Mariner-like elements (MLEs) are common DNA transposons found in animal genomes.
  • In vitro transposition requires only transposase, but host, environmental, and transposon sequence factors can affect in vivo transposition.

Purpose of the Study:

  • To investigate factors influencing the transposition of Mos1, a Mariner-like element.
  • To demonstrate in vitro transposition of MITEs (Miniature Inverted-repeat Transposable Elements) derived from MLEs.
  • To explore the impact of experimental conditions on MLE transposition.

Main Methods:

  • In vitro transposition assays using Mos1 and MLE-derived MITEs.
  • Analysis of DNA stuffer sequence (GC content, length) and super-helicity effects.
  • Temperature variation to assess transposition regulation.

Main Results:

  • Mos1 transposition efficiency depends on DNA stuffer GC content and length.
  • Successfully demonstrated in vitro transposition of small MITEs (80-120 bp).
  • High temperatures reduce Mos1 transposition, leading to non-integrating products; DNA super-helicity significantly impacts efficiency.

Conclusions:

  • Experimental conditions can significantly alter the interpretation of mariner excision frequency and quality.
  • The auto-integration pathway limits in vitro transposition efficiency to new sites, a phenomenon potentially relevant in natural hosts.
  • A model for small transposon transposition that circumvents DNA bending constraints is proposed.

More Related Videos

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay14:34

Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay

Published on: December 25, 2021

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides07:25

Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides

Published on: December 26, 2010

Related Concept Videos

Characteristics of MOSFET01:17

Characteristics of MOSFET

Metal-oxide-semiconductor field-effect Transistors, or MOSFETs, play a critical role in electronic circuits. They are primarily utilized for amplifying and switching signals.
Various vital parameters influence their functionality, which is crucial for theory and electronics applications. First, channel dimensions, precisely length, and width, are pivotal. The size of these channels affects the transistor's ability to carry current and switching speeds; shorter channels typically enable quicker...
Overview of Transposition and Recombination02:13

Overview of Transposition and Recombination

Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
MOSFET: Enhancement Mode01:22

MOSFET: Enhancement Mode

Enhancement-mode MOSFETs are pivotal components in electronics, distinguished by their capacity to act as highly efficient switches. They are part of the larger family of metal-oxide Semiconductor Field-Effect Transistors (MOSFETs). They are available in two types: p-channel and n-channel, each tailored to specific polarity operations.
In their basic form, enhancement-mode MOSFETs are typically non-conductive when the gate-source voltage (Vgs) is zero. This default 'off' state means no current...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transposons01:24

Transposons

Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
Energy to Drive Translocation01:37

Energy to Drive Translocation

Mitochondrial protein import is powered by two distinct energy sources: ATP hydrolysis and electrochemical potential across the inner membrane. Newly synthesized precursors are bound by cytosolic chaperones of the Hsp70 family, which guide them to the import receptors on the mitochondrial surface. Utilizing the energy of ATP hydrolysis, Hsp70 chaperones transfer these precursors to the TOM receptors on the mitochondrial outer membrane.
Generally, polypeptides are unfolded by two distinct...