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Updated: Jun 27, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
Published on: January 20, 2022
A method for monitoring and controlling reproducibility of intensity data in complex electrospray mass spectra: a
Paolo Lecchi1, Jinghua Zhao, Wesley S Wiggins
1Correlogic Systems, Inc., Rockville, Maryland 20850, USA.
Abstract:
Reproducibility in mass spectral data is important in both biomarker discovery and spectral database searching. We report a strategy, employing a series of substituted benzylpyridinium thermometer ions that can be used to monitor changes in performance of multiple aspects of an electrospray ionization source that impact the intensity axis of a spectrum. Performance attributes, which could confound even isotope-based quantification strategies, are readily assessed using a mixture of thermometer ions. Based on the observed behavior of the ions, a procedure is proposed for monitoring instrument performance and compensating for factors that affect reproducibility across both time and instruments.
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