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Updated: Jun 27, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

Factors affecting hematopoietic progenitor cell mobilization: an analysis of 307 patients

Alfredo Mendrone1, Cyntia Araujo Arrais, Rosaura Saboya

  • 1Fundação Pró-Sangue/Hemocentro de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 155 - 1 degrees andar, Cerqueira Cesar, São Paulo, SP 05403-000, Brazil. alfredo.mendrone@prosangue.sp.gov.br

Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis
|November 28, 2008
PubMed

We reviewed the data of 307 patients treated with autologous bone marrow transplantation with the aim to identify factors associated with poor hematopoietic stem cell (HSC) mobilization after administration of cyclophosphamide and granulocyte-colony stimulating factor. Success in mobilization was defined when > or = 2.0 x 10(6) CD34+ cells/kg weight could be collected with < or = 3 leukapheresis procedures. Success was observed in 260 patients (84.7%) and nonsuccess in 47 patients (15.3%). According to the stepwise regression model: diagnosis, chemotherapy load, treatment with mitoxantrone and platelet count before mobilization were found to be independent predictive factors for HSC mobilization. These results could help in the previous recognition of patients at risk for non response to mobilization and allow to plan an alternative protocol for this group of patients.

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