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Pil1 controls eisosome biogenesis.

Karen E Moreira1, Tobias C Walther, Pablo S Aguilar

  • 1Howard Hughes Medical Institute, and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA 94158, USA.

Molecular Biology of the Cell
|November 28, 2008
PubMed
Summary
This summary is machine-generated.

Eisosomes, protein assemblies in yeast plasma membranes, form de novo in dividing cells. Pil1 protein regulates their size and location, synchronized with cell division for proper membrane growth.

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Published on: July 26, 2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Yeast Genetics

Background:

  • Plasma membranes are dynamic structures remodeled by endocytosis and exocytosis.
  • Eisosomes are large, immobile protein assemblies on the yeast plasma membrane.
  • Eisosome disruption causes plasma membrane invaginations and endocytic defects.

Purpose of the Study:

  • To investigate the formation, inheritance, size, distribution, and location of eisosomes.
  • To elucidate the molecular mechanisms governing eisosome biogenesis and regulation.

Main Methods:

  • Observation of de novo eisosome formation in dividing yeast cells.
  • Analysis of eisosome colonization patterns on newly formed membrane.
  • Investigation of the role of Pil1 protein in eisosome biogenesis.
  • Correlation of Pil1 expression with cell cycle and plasma membrane growth.

Main Results:

  • Eisosomes form de novo in yeast buds, colonizing new membrane in a polarized wave.
  • Pil1 protein is a key regulator of eisosome size and location.
  • Pil1 levels dictate eisosome size and density, with minimal size and limited nucleation sites observed.
  • Cell cycle-regulated Pil1 expression synchronizes eisosome formation with membrane growth.

Conclusions:

  • Established a framework for eisosome assembly and distribution principles.
  • Demonstrated de novo formation and polarized colonization of eisosomes.
  • Identified Pil1 as a central regulator of eisosome biogenesis, linking it to cell cycle and membrane dynamics.