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Structural analysis by circular dichroism of some enzymes involved in plant cell wall degradation
P W Goodenough1, D C Clark, A J Durrant
1Department of Protein Engineering Reading Laboratory, AFRC Institute of Food Research, UK.
FEBS Letters
|May 6, 1991
Abstract:
Three enzymes which degrade different polysaccharide components of plant cell walls have been characterized by circular dichroism (CD). A bacterial endoglucanase, which in the native state forms part of a multiprotein cellulase complex, showed a tendency to form aggregates as measured by CD. Depending on its degree of aggregation, this enzyme displayed between 50% and 100% helical structure, whereas a bacterial xylanase and a fungal polygalacturonase exhibited more beta-sheet structure. The polygalacturonase was apparently devoid of helical structure.