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Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
Published on: November 8, 2016
Regulatory T cells control VEGF-dependent skin inflammation
Ingrid Teige1, Henning Hvid, Lars Svensson
1Department of Pharmacology, Section of Dermatology, Discovery, LEO Pharma A/S, Ballerup, Denmark.
The Journal of Investigative Dermatology
|November 28, 2008
Summary
Regulatory T cells suppress inflammation in a mouse model of psoriasis. Transferring these cells reduced skin inflammation and cytokine levels, suggesting a therapeutic potential for treating psoriasis.
Area of Science:
- Immunology
- Dermatology
- Genetics
Background:
- Transgenic mice expressing vascular endothelial growth factor (VEGF) under the keratin 14 promoter exhibit psoriasis-like skin inflammation.
- 12-O-tetradecanoylphorbol-13-acetate (TPA) application in these mice exacerbates inflammation, creating a Th17 cell signature.
Purpose of the Study:
- To investigate the function of CD4(+) T cells in a TPA-induced psoriasis-like skin inflammation model.
- To determine if regulatory T cells can ameliorate this specific type of skin inflammation.
Main Methods:
- Utilized K14/VEGF transgenic mice and TPA application to induce skin inflammation.
- Depleted CD4(+) T cells using antibody treatment to assess their role.
- Transferred sorted regulatory CD4(+)CD25(+) T cells into naive mice prior to TPA challenge.
Main Results:
- CD4(+) T cell depletion worsened ear thickness and proinflammatory cytokine levels.
- An increased proportion of regulatory T cells (CD4(+)CD25(+)CD127(-/low)) was observed in inflamed skin.
- Regulatory T-cell transfer significantly reduced ear thickness and cytokine production.
Conclusions:
- CD4(+) T cells exert a suppressive function in this psoriasis model.
- Regulatory T cells demonstrate therapeutic potential for controlling persistent skin inflammation resembling psoriasis.
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