Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

IR Spectroscopy: Molecular Vibration Overview

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

Two-Dimensional (2D) NMR: Overview

Two-Dimensional (2D) NMR: Overview

The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Donor bone marrow together with recipient regulatory T cells induces chimerism without irradiation in kidney transplantation.

Science translational medicine·2026

Same author

Disentangling Single- and Biexciton Dynamics with Photoelectron-Detected Two-Dimensional Electronic Spectroscopy.

The journal of physical chemistry letters·2026

Same author

Comparison of sodium and potassium measurements in the emergency department by point-of-care vs. central laboratory testing : A retrospective analysis.

Wiener klinische Wochenschrift·2026

Same author

Hypokalaemia in the emergency department: aetiology, diagnosis, and management.

Swiss medical weekly·2026

Same author

Simultaneous microwave ablation and liver resection in patients with colorectal liver metastases.

HPB : the official journal of the International Hepato Pancreato Biliary Association·2026

Same author

Spontaneous transanal evisceration of the small bowel through perforation of the sigmoid colon: a case report and new surgical approach.

International journal of surgery case reports·2026

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Inherently phase-stable coherent two-dimensional spectroscopy using only conventional optics.

Ulrike Selig¹, Florian Langhojer, Frank Dimler

¹Institut für Physikalische Chemie, Universität Würzburg, Am Hubland, 97074 Würzburg, Germany.

|November 28, 2008

Summary

This summary is machine-generated.

We developed a simple, phase-stable setup for two-dimensional femtosecond spectroscopy. This robust method avoids complex optics and works across a wide range of light frequencies.

More Related Videos

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Published on: July 17, 2016

Related Experiment Videos

Last Updated: Jun 27, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Published on: July 17, 2016

Area of Science:

Physical Sciences
Spectroscopy
Ultrafast Optics

Background:

Coherent two-dimensional (2D) spectroscopy is a powerful technique for probing molecular dynamics.
Previous setups often require complex and expensive components like diffractive optics or active phase-locking systems.
These complexities can limit the robustness and applicability of 2D spectroscopy.

Purpose of the Study:

To develop a simplified and inherently phase-stable setup for coherent two-dimensional femtosecond spectroscopy.
To demonstrate a robust and versatile spectroscopy system that avoids specialized optical components.
To enable broadband 2D spectroscopy across infrared, visible, and ultraviolet spectral ranges.

Main Methods:

Implementation of a noncollinear box geometry for 2D spectroscopy.
Utilization of only conventional optical components: beam splitters, mirrors, and delay stages.
Avoidance of diffractive optics, pulse shapers, and active phase-locking loops.

Main Results:

Achieved an inherently phase-stable setup for coherent 2D femtosecond spectroscopy.
Demonstrated a simple and robust experimental configuration.
Verified applicability for ultrabroad bandwidths, covering infrared, visible, and ultraviolet spectral regimes.

Conclusions:

The proposed setup offers a simplified and cost-effective approach to coherent 2D femtosecond spectroscopy.
Its robustness and broad spectral applicability make it suitable for diverse research areas.
This advancement facilitates advanced spectroscopic studies of molecular dynamics without complex instrumentation.