Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Published on: August 10, 2016

Using both xylanase and laccase enzymes for pulp bleaching.

Cristina Valls¹, M Blanca Roncero

¹Textile and Paper Engineering Department, ETSEIAT, Universitat Politècnica de Catalunya, Colom 11, E-08222 Terrassa, Spain.

Bioresource Technology

|November 29, 2008

Summary

Xylanase pretreatment enhances kraft pulp bleaching efficiency. Combining xylanase, laccase, and alkaline extraction significantly reduces enzyme and mediator use for achieving target brightness.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From mold to mill: StachCel5, a novel thermoalkaliphilic endoglucanase from Stachybotrys chartarum for pulp fiber biorefining.

International journal of biological macromolecules·2025

Same author

Periodate oxidation of nanofibrillated cellulose films for active packaging applications.

International journal of biological macromolecules·2024

Same author

Composites of cellulose nanocrystals in combination with either cellulose nanofibril or carboxymethylcellulose as functional packaging films.

International journal of biological macromolecules·2022

Same author

Sustainability in e-commerce packaging: A review.

Journal of cleaner production·2020

Same author

Development of an antimicrobial bioactive paper made from bacterial cellulose.

International journal of biological macromolecules·2020

Same author

Bacterial Cellulose-Chitosan Paper with Antimicrobial and Antioxidant Activities.

Biomacromolecules·2020

Area of Science:

Biotechnology
Pulp and Paper Science
Green Chemistry

Background:

Oxygen-delignified eucalyptus kraft pulp requires bleaching to achieve desired brightness.
Conventional bleaching methods can be environmentally intensive.
Enzymatic treatments offer a potentially greener alternative for pulp bleaching.

Purpose of the Study:

To evaluate the efficacy of a combined xylanase-laccase-alkaline extraction (XLE) sequence for bleaching eucalyptus kraft pulp.
To compare the XLE sequence with a laccase-alkaline extraction (LE) sequence.
To optimize the laccase-mediator system conditions and assess the impact of xylanase pretreatment.

Main Methods:

Enzymatic treatments using xylanase (X) and laccase (L) in an XLE sequence.

More Related Videos

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes

Published on: December 12, 2013

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

Related Experiment Videos

Last Updated: Jun 27, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids

Published on: August 10, 2016

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes

Published on: December 12, 2013

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization

Published on: October 24, 2011

Comparison with an LE sequence for bleaching oxygen-delignified eucalyptus kraft pulp.

Optimization of laccase-mediator (1-hydroxybenzotriazol, HBT) system using a sequential statistical plan.

Main Results:

The XLE sequence demonstrated improved bleaching performance compared to the LE sequence.
Optimized laccase-mediator system conditions involved a reduced mediator dose (0.5% odp).
Xylanase pretreatment enhanced lignin accessibility and hexenuronic acid removal, leading to significant reductions in laccase (30%), mediator (80%), and reaction time (45%) for 70% ISO brightness.

Conclusions:

Xylanase pretreatment is highly effective in improving the efficiency of laccase-mediated bleaching.
The XLE sequence offers substantial savings in enzyme and mediator consumption.
This enzymatic approach provides a more sustainable and cost-effective method for achieving high brightness in kraft pulp.