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Updated: Jun 27, 2026

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
Published on: February 16, 2022
Enzyme-linked immunosorbent assay method for detection of cytomegalovirus strain-specific antibody responses
Zdenek Novak1, Shannon A Ross, Raj Kumar Patro
1Department of Pediatrics, The University of Alabama at Birmingham, Birmingham, Alabama 35233, USA. zdnovak@peds.uab.edu
Abstract:
Reliable methods for the detection of cytomegalovirus (CMV) strain-specific serological responses are lacking. We describe a simple and reliable enzyme-linked immunosorbent assay method developed to detect antibodies against the polymorphic epitopes within the two envelope glycoproteins of CMV, glycoproteins H and B. This assay is useful for the detection of serologic responses to CMV strains and the identification of CMV reinfections.
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Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

