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Related Experiment Video

Updated: Jun 27, 2026

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

The IGF system in in-vitro human decidualization.

C Ganeff¹, G Chatel, C Munaut

¹Laboratory of Tumor and Development Biology, Center of Experimental Cancer Research, University of Liège, GIGA-R, B-4000 Liège, Belgium.

Molecular Human Reproduction

|November 29, 2008

Summary

The insulin-like growth factor (IGF) network is involved in decidualization, a key process for pregnancy. Researchers found that IGF-II and its receptors are upregulated, suggesting IGF signaling plays a crucial role in endometrial cell function.

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Last Updated: Jun 27, 2026

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Isolation of Human Endometrial Stromal Cells for In Vitro Decidualization

Published on: September 1, 2018

Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization

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Published on: March 2, 2017

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae

Published on: April 30, 2018

Area of Science:

Reproductive biology
Cellular signaling
Endocrinology

Background:

Decidualization of endometrial stromal cells (ESCs) is essential for pregnancy.
The molecular mechanisms underlying decidualization are not fully understood.

Purpose of the Study:

To investigate the role of the insulin-like growth factor (IGF) network in human ESC decidualization.
To examine the expression and activation of IGF system components during in-vitro decidualization.

Main Methods:

Cultured human ESCs were induced to undergo in-vitro decidualization.
mRNA and protein levels of IGF system components were analyzed.
Phosphorylation status of receptors and adaptor proteins was assessed.

Main Results:

Significant upregulation of IGF-II, IGF-I receptor, and insulin receptor (InsR) isoforms A and B was observed.
Levels of adaptor proteins insulin receptor substrate 1 (IRS-1) and IRS-2 increased.
IRS-2 showed slight phosphorylation, further activated by IGF-II, indicating pathway activation.

Conclusions:

The IGF signaling pathway is likely involved in the decidualization of endometrial stromal cells.
Components of the IGF system, including IGF-II and its receptors, are dynamically regulated during decidualization.
Activated IGF signaling may play a crucial role in the functions of decidualized endometrial cells.