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Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...

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Adapting a denitrifying biocathode for perchlorate reduction.

Caitlyn Shea1, Peter Clauwaert, Willy Verstraete

  • 1Department of Civil Engineering and Geological Science, University of Notre Dame, Notre Dame, IN 46556, USA. cshea1@nd.edu

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|November 29, 2008
PubMed
Summary

Dissimilatory perchlorate-reducing bacteria (PCRB) can treat perchlorate contamination by using a microbial fuel cell (MFC) cathode as an electron donor. This study confirms PCRB can function without added electron shuttles, offering a novel bioremediation strategy.

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Area of Science:

  • Environmental Science
  • Microbiology
  • Electrochemistry

Background:

  • Perchlorate is an environmental contaminant due to its use in aerospace and defense industries.
  • Perchlorate inhibits thyroid function and is highly mobile in water.
  • Bioreduction to chloride by dissimilatory perchlorate-reducing bacteria (PCRB) is a promising remediation strategy.

Purpose of the Study:

  • To investigate perchlorate reduction using a microbial fuel cell (MFC) with a denitrifying biocathode.
  • To confirm if PCRB can utilize the cathode as an electron donor without exogenous electron shuttles.

Main Methods:

  • A functioning MFC with a denitrifying biocathode was employed.
  • Perchlorate concentration was gradually increased while nitrate concentration was decreased.
  • Perchlorate reduction rates and MFC performance were monitored.

Main Results:

  • The MFC achieved a maximum perchlorate removal rate of 12 mg/L-d.
  • Perchlorate reduction contributed 64% to the cell's electrical output.
  • Perchlorate reduction efficiency improved over time, indicating adaptation.

Conclusions:

  • PCRB can effectively utilize the MFC cathode as an electron donor for perchlorate bioremediation.
  • This process can occur without the need for external electron shuttles.
  • MFCs offer a viable technology for in-situ perchlorate remediation.